Antibacterial activity of silver nanoparticles and lemon peel mixture on E-coli Bacteria
Maryam M. Kadhim
Baida M. Ahmed
DOI: https://doi.org/10.47831/mjpas.v1i1.12
Keywords: silver nanoparticles, a new method to prepare the extract, lemon peel extract, inhibition E. coli bacteria
Abstract
In this work, pulsed laser ablation in liquid (PLAL) technique is used to prepare the Ag nanoparticles in water, and prepare lemon peel extract by plasma discharge in a fabricated DC plasma jet in record time at two intervals (5,15) minutes, using Nd:YAG laser wavelength 532 nm at three energies (500, 700, and 900) mJ. Where mixed the colloidal Ag nanoparticles with lemon peel extract in different conditions and applied it on the antibacterial activity Escherichia coli (E. coli.). The antibacterial activity was performed by the agar disc diffusion method. The study confirmed that there was a significant improvement in bacterial inhibition in the case of mixing. Study the UV before and after mixing we note increases in intensity. the inhibition increasing with energy, the best inhibition take place at 900 mJ, at 15 minutes.