Detection of virulence factors of bacterial isolates associated with Acute Myeloid Leukemia (AML) and Acute Lymphocytic Leukemia (ALL) patients

Abstract

Samples include (blood ,urine , pus ,skin and throat swab) were taken from 200 leukemia patients who attended to Hematology and transplantation center in medical city complex. The bacterial isolates were identified by phenotypic examination, biochemical tests, as well as VITEK-2 assay. gram positive isolates were most prevalent than gram negative,  Staphylococcus epidermidis was the prevalent bacteria isolated from different source samples of leukemia patients followed by Staphylococcus aureus and klebsiella and E.coli among gram negative. The bacteria isolated in our study demonstrated significant resistance to commonly used antibiotics, with most classified as MDR and others as PDR. Interestingly, Synergistic effects were detected among certain antibiotics against bacteria isolated from various sources. Most isolates hadn't hemolytic activity and the rest showed beta hemolysis expect Micrococcus luteus and Enterobacter cloacae showed alpha hemolysis. . Most isolates from numerous bacterial groups showed strong and moderate  biofilm production.