Evaluation antibacterial Efficacy of different Calcined Temperature of the MgO NPS Prepared by Hydrothermal Methods Against Serratia. marcescens Isolated From Post-Operative Wound Infection

Abstract

The main aim of this study was to develop a straightforward procedure for synthesizing magnesium oxide nanoparticles (MgO NPs) that are used as antimicrobial agents against Serratia.marcescens, gram negative bacteria were isolated from post-operative wound infection.   A high-purity MgO NPS  has been prepared by applying different calcinations temperatures (200ºC, 400ºC, 650ºC) using the hydrothermal chemical method. The hydrothermal technique is a process used to create a crystalline nanomaterial’s from  high-temperature aqueous solutions with high vapour pressures. This way can be prepared more shape of nanoparticles that are highly stable. The structural, morphological and optical  properties ‘of MgO NPs were conducted using : XRD, FE-SEM and UV-vis  analysis respectively. The crystallinity it increases with an increase of calcination temperature that reducing the effect on antibacterial activity were done on pathogenic bacterium as result of increase in particle size with sharp intense peak. The antimicrobial effects of the prepared MgO NPs were examined against MDR strains using Micro-dilution titer method with various doses of  minimum inhibitory concentration (MIC) of MgO NPs (1500, 1000, 500, 250, 125, 62.5, 31.5 µg/ml^-1 ). The higher value was decreased the bacterial growth was recorded at 1500µg/ml^-1concentration at 650 ºC, and the lower value was recorded as 31.5 µg/ml^-1 concentration at 200ºC. This preparation procedure yielded a higher nanoparticles effectiveness  in  fast, simple and economical manner that have active role against microbial growth.