Mustansiriyah Journal of Pure and Applied Sciences

Bactericidal effects of silver nanoparticles prepared by green synthesis

Intesar H. Hashim

Mustansiriyah University

Zainab Saad Mahdi

Nadia M Jassim

DOI: https://doi.org/10.47831/mjpas.v2i4.80

Keywords: AgNPs, Green synthesis, Escherichia coli, SEM, XRD, AFM

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Abstract

This work aims to paper silver nanoparticles (AgNPs) using the green synthesis method, considered a low-cost and environmentally friendly method, and to study the structural properties of these nanoparticles by X-ray diffraction (XRD), scanning electron microscope (SEM), and atomic force microscopy (AFM) measurements. The results of XRD show that the AgNPs are polycrystalline in the dominant direction (122) (111), (200) (220) (311). By studying the surface morphology, the AFM results showed that the average particle size of the AgNPs is (6.73 nm). In contrast, the SEM results showed that the AgNPs have a spherical shape, and the average particle size is (52.532 nm). The chemical signature of AgNPs was known through FTIR measurement and was found to consist of a group of organic compounds. Through biological application, AgNPs were found to be effective in killing Escherichia coli, which is usually present in contaminated water and infects the intestines of humans and animals. That allows the AgNPs to effectively reduce many human diseases caused by these types of bacteria.